Estimating fine root biomass with DNA fingerprints

Research Issue

Aspen stands of the Aspen FACE experiment were established as a mixture of five clones, so development of highly discriminating molecular-methods were needed to assign samples of fine-root fragments to individual clones.

Our Research

Young stands of trembling aspen (Populus tremuloides) were grown under elevated concentrations of carbon dioxide and elevated ozone in the Aspen FACE experiment at Harshaw, WI between 1997 and 2009. Microsatellite markers are being developed to generate distinct DNA fingerprints for each of the five-aspen clones. These DNA fingerprints will be used to quantify fine-root biomass from the below ground portion of the final 2009 analytical harvest. In addition, the markers will be used to identify and quantify clonal regeneration response and development following the 2009 harvest.

Expected Outcomes

Biomass determination for each aspen genotype in the Aspen FACE experiment and identification of clonal response in natural regeneration following harvest will contribute knowledge needed for the management of forested lands under exposure to atmospheric pollutants.

Research Participants

Principal Investigators

• Paula Marquardt, US Forest Service Northern Research Station - Research Plant Geneticist
• Mark Kubiske, US Forest Service Northern Research Station - Research Plant Physiologist