Evolutionary consequences of putative intra- and interspecific hybridiation in agaric fungi
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Mycologia. 105(6): 1577-1594.
Agaric fungi of the southern Appalachian Mountains including Great Smoky Mountains National Park are often heterozygous for the rDNA internal transcribed spacer region (ITS) with .42% of collections showing some heterozygosity for indels and/or base-pair substitutions. For these collections, intra-individual haplotype divergence is typically less than 2%, but for 3% of these collections intra-individual haplotype divergence exceeds that figure. We hypothesize that high intra-individual haplotype divergence is due to hybridization between agaric fungi with divergent haplotypes, possibly migrants from geographically isolated glacial refugia. Four species with relatively high haplotype divergence were examined: Armillaria mellea, Amanita citrina f. lavendula, Gymnopus dichrous and the Hygrocybe flavescens/chlorophana complex. The ITS region was sequenced, haplotypes of heterozygotes were resolved through cloning, and phylogenetic analyses were used to determine the outcome of hybridization events. Within Armillaria mellea and Amanita citrinalavendula, we found evidence of interbreeding and recombination.
Keywordsbiodiversity; Dobzhansky-Muller incompatibility; hybridization; speciation
Hughes, Karen W.; Petersen, Ronald H.; Lodge, D. Jean; Bergemann, Sarah E.; Baumgartner, Kendra; Tulloss, Rodham E.; Lickey, Edgar; Cifuentes, Joaquin. 2013. Evolutionary consequences of putative intra- and interspecific hybridiation in agaric fungi. Mycologia. 105(6): 1577-1594. https://doi.org/10.3852/13-041.