White oak (Quercus alba L.) microsatellite markers for genetic diversity studies
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SRS Research Note-SRS-26. Asheville, NC: U.S. Department of Agriculture Forest Service, Southern Research Station.
We screened and validated microsatellite DNA markers (i.e., simple sequence repeats, SSRs) from transcriptome sequences of white oak (Quercus alba L.). Of 84 PCR primer pairs previously identified and designed, we found 23 pairs that amplified white oak genomic DNA consistently and were polymorphic among three DNA samples. Subsequently, 16 of these 23 primer pairs were amplified across 225 white oak trees sampled from naturally regenerated stands on the Daniel Boone National Forest in southeastern Kentucky. Population estimates of heterozygosity and total and effective allele numbers averaged 66.4%, 11.3, and 5.5, respectively. These 16 SSR markers proved to be highly polymorphic and provided informative data on the genetic diversity of the white oaks within these stands.
KeywordsForest genetics; gene diversity; molecular markers; population genetics; silvicultural thinning
Thunder, Rachel; Conrad, Anna O.; Burdine, Charles; Yang, Jian; Lhotka, John M.; Abbott, Albert G.; Nelson, C. Dana. 2022. White oak (Quercus alba L.) microsatellite markers for genetic diversity studies. Res. Note SRS-26. Asheville, NC: U.S. Department of Agriculture Forest Service, Southern Research Station. 6 p. https://doi.org/10.2737/SRS-RN-26.