Publication Details

Fungal endophytes and origins of decay in beech (Fagus sylvatica) sapwood

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Gilmartin, Emma C.; Jusino, Michelle; Pyne, Edward J.; Banik, Mark T.; Lindner, Daniel L.; Boddy, Lynne

Year Published

2022

Publication

Fungal Ecology

Abstract

Sapwood comprises much above-ground forest biomass, but its mycobiome in living trees is largely unknown. Here, we characterize the endophytic fungal communities of the functional sapwood of young and mature living beech trees (Fagus sylvatica) at multiple scales, from within individual trees to woodland sites across the southern United Kingdom. Fungal community composition was determined using both culture-based and molecular approaches across two loci. Wood decay fungi, including those that cause heart rot, were detected in approximately 80% of all samples. Fungal community composition differed according to the survey approach (high throughput sequencing vs. isolation of fungi into culture) and between geographic location and individual trees, but no significant patterns were detected at different heights in individual trees or around their circumferences. ITS and LSU sequencing detected more distinct taxa than culturing. However, LSU primers yielded more OTUs than did ITS primers, though both identified unique OTUs. This highlights the importance of multiple survey approaches, including multiple primer pairs, for better characterisation of communities and confidence in results of endophyte studies.

Keywords

HTS; Metabarcoding; Fungal guilds; Latent fungi; Wood saprotroph; Decay fungi; Mycobiome; Xylem

Citation

Gilmartin, Emma C.; Jusino, Michelle A.; Pyne, Edward J.; Banik, Mark T.; Lindner, Daniel L.; Boddy, Lynne. 2022. Fungal endophytes and origins of decay in beech (Fagus sylvatica) sapwood. Fungal Ecology. 59: 101161. 9 p. https://doi.org/10.1016/j.funeco.2022.101161.

Last updated on: September 13, 2022