Nondormancy in Entomophaga maimaiga azygospores: effects of isolate and cold exposure
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Mycologia. 100(6): 833-842.
Azygospores (resting spores) of the fungal pathogen Entomophaga maimaiga are produced in later larval instars of the gypsy moth Lymantria dispar and normally enter constitutive dormancy. In the laboratory cadavers of recently dead larvae containing immature azygospores were placed on 1.0% water agar at 20 C for 2 wk after host death, allowing time for azygospore maturation. We found that some azygospores produced in this way did not enter dormancy. To investigate nondormancy, suspensions of azygospores from individual cadavers were transferred to moist, sterile soil at 15 C. Groups of gypsy moth larvae were exposed sequentially to azygospores for consecutive 4 d periods for 196 d. Infections first were seen among larvae exposed 24?28 d after bioassays began, and infection potential continued 196 d.
Keywordsbiological control; Entomophthorales; gypsy moth; Lymantria dispar; spore dormancy; Zygomycota
Hajek, Ann E.; Burke, Allison E.; Nielsen, Charlotte; Hannam, Joshua J.; Bauer, Leah S. 2008. Nondormancy in Entomophaga maimaiga azygospores: effects of isolate and cold exposure. Mycologia. 100(6): 833-842. https://doi.org/10.3852/07-160.